PhIP can accurately identify known and previously unreported Coursework

PhIP can accurately identify known and previously unreported autoantigens - Coursework Example The paper "PhIP can accurately identify known and previously unreported autoantigens" seeks to affirm the hypothesis that â€Å"PhIP can accurately identify known and previously unreported autoantigens† figure 3 shows seven figure that represent various experimental processes. Key technical issues in the program of work include the accurate and PhIP sequencing and T7 cloning, verifying and analyzing results from human samples with different consenting PND patients. Firstly the human protein and cDNA sequences available are downloaded from the database at build 35.1 of the human genome. Then this cDNA and the proteins are used to construct the library. This procedure involved the proteome wide library and the N/C terminal libraries that were PCR amplified as 23 independent pools with common primer sequences. This process was conducted in a controlled thermal profile. The polymerase chain reaction was then digested and cloned into the T7FNS2 making numerous copies of each peptid e.the cloning process involved the inserting a peptide in the required ORF sequence selected using restriction enzymes. This part is an a explanation of the processes involved in fig 3.d, g, f, c, e Patient A, B and C samples are collected from willing patients approved by relevant medical authorities. The samples of cerebrospinal fluids were kept at temperatures of - 80 degrees Celsius before being used. Before the collection of sample the patients have to undergo various neurological evaluations before the samples.